SDS Page:Article Title: The derlin Dfm1 couples retrotranslocation of a folded protein domain to its proteasomal degradation.
Article Snippet: .. Protein samples were analyzed by SDS-PAGE on 4–20% gradient Criterion TGX precast gels (Bio-Rad), transferred onto a PVDF membrane, and incubated with the indicated antibodies. .. The signal was analyzed by enhanced chemiluminescence (Western Lightning ECL Pro, Perkin Elmer), visualized with Amersham Imager 600 (GE Healthcare Life Sciences), and quantified using Vitali et al. Journal of Cell Biology 12 of 16 Retrotranslocation of folded domains https://doi.org/10.1083/jcb.202308074 D ow nloaded from http://rupress.org/jcb/article-pdf/223/5/e202308074/1924956/jcb_202308074.pdf by guest on 23 April 2024 Image Studio software (Li-Cor).
Article Title: Synaptopodin enables directional mechanoadaptation of integrin-based adhesions
Article Snippet: .. Proteins were resolved on 4–20% gradient Criterion TGX precast gels (Bio-Rad #5671095) by SDS-PAGE at 125 V for 1 hour, then transferred to nitrocellulose membranes (Bio-Rad #1620112) using a wet transfer system at 40 V for 2 hours at 4°C. .. Membranes were blocked with 5% non-fat dry milk in PBST (PBS containing 0.2% Tween-20; Fisher BioReagents, BP337-500) for 30 minutes at room temperature with gentle agitation.
Membrane:Article Title: The derlin Dfm1 couples retrotranslocation of a folded protein domain to its proteasomal degradation.
Article Snippet: .. Protein samples were analyzed by SDS-PAGE on 4–20% gradient Criterion TGX precast gels (Bio-Rad), transferred onto a PVDF membrane, and incubated with the indicated antibodies. .. The signal was analyzed by enhanced chemiluminescence (Western Lightning ECL Pro, Perkin Elmer), visualized with Amersham Imager 600 (GE Healthcare Life Sciences), and quantified using Vitali et al. Journal of Cell Biology 12 of 16 Retrotranslocation of folded domains https://doi.org/10.1083/jcb.202308074 D ow nloaded from http://rupress.org/jcb/article-pdf/223/5/e202308074/1924956/jcb_202308074.pdf by guest on 23 April 2024 Image Studio software (Li-Cor).
Article Title: O -Linked N -Acetylglucosamine ( O -GlcNAc) Transferase and O -GlcNAcase Interact with Mi2β Protein at the A γ-Globin Promoter
Article Snippet: .. Immunoblotting All electrophoresis was performed with 4–15% gradient Criterion TGX precast gels (567-1084, Bio-Rad) at 140 V followed by transfer of protein to PVDF membrane (IPVH00010, Millipore) at 0.4 A. Blots were blocked by 3% BSA in TBST (25 m m Tris-HCl, pH 7.6, 150 m m NaCl, and 0.05% Tween 20) at room temperature for 20 min and then incubated with primary antibody at 4 °C overnight. .. After washing with TBST five times for 5 min each time, blots were incubated with HRP-conjugated secondary antibody for 1 h at room temperature followed by TBST washes again and developed using chemiluminescent substrate (E2400, Denville Scientific).
Article Title: O-Linked N-Acetylglucosamine (O-GlcNAc) Transferase and O-GlcNAcase Interact with Mi2β Protein at the Aγ-Globin Promoter
Article Snippet: .. Immunoblotting: All electrophoresis was performed with 4-15% gradient Criterion TGX Precast Gels (567-1084, Bio-Rad) at 140 volts, followed by transferring protein to PVDF membrane (IPVH00010, Millipore) at 0.4 Amps. ..
Article Title: O -Linked N -Acetylglucosamine ( O -GlcNAc) Transferase and O -GlcNAcase Interact with Mi2β Protein at the A γ-Globin Promoter
Article Snippet: .. All electrophoresis was performed with 4–15% gradient Criterion TGX precast gels (567-1084, Bio-Rad) at 140 V followed by transfer of protein to PVDF membrane (IPVH00010, Millipore) at 0.4 A. Blots were blocked by 3% BSA in TBST (25 m m Tris-HCl, pH 7.6, 150 m m NaCl, and 0.05% Tween 20) at room temperature for 20 min and then incubated with primary antibody at 4 °C overnight. .. After washing with TBST five times for 5 min each time, blots were incubated with HRP-conjugated secondary antibody for 1 h at room temperature followed by TBST washes again and developed using chemiluminescent substrate (E2400, Denville Scientific).
Incubation:Article Title: The derlin Dfm1 couples retrotranslocation of a folded protein domain to its proteasomal degradation.
Article Snippet: .. Protein samples were analyzed by SDS-PAGE on 4–20% gradient Criterion TGX precast gels (Bio-Rad), transferred onto a PVDF membrane, and incubated with the indicated antibodies. .. The signal was analyzed by enhanced chemiluminescence (Western Lightning ECL Pro, Perkin Elmer), visualized with Amersham Imager 600 (GE Healthcare Life Sciences), and quantified using Vitali et al. Journal of Cell Biology 12 of 16 Retrotranslocation of folded domains https://doi.org/10.1083/jcb.202308074 D ow nloaded from http://rupress.org/jcb/article-pdf/223/5/e202308074/1924956/jcb_202308074.pdf by guest on 23 April 2024 Image Studio software (Li-Cor).
Article Title: O -Linked N -Acetylglucosamine ( O -GlcNAc) Transferase and O -GlcNAcase Interact with Mi2β Protein at the A γ-Globin Promoter
Article Snippet: .. Immunoblotting All electrophoresis was performed with 4–15% gradient Criterion TGX precast gels (567-1084, Bio-Rad) at 140 V followed by transfer of protein to PVDF membrane (IPVH00010, Millipore) at 0.4 A. Blots were blocked by 3% BSA in TBST (25 m m Tris-HCl, pH 7.6, 150 m m NaCl, and 0.05% Tween 20) at room temperature for 20 min and then incubated with primary antibody at 4 °C overnight. .. After washing with TBST five times for 5 min each time, blots were incubated with HRP-conjugated secondary antibody for 1 h at room temperature followed by TBST washes again and developed using chemiluminescent substrate (E2400, Denville Scientific).
Article Title: O -Linked N -Acetylglucosamine ( O -GlcNAc) Transferase and O -GlcNAcase Interact with Mi2β Protein at the A γ-Globin Promoter
Article Snippet: .. All electrophoresis was performed with 4–15% gradient Criterion TGX precast gels (567-1084, Bio-Rad) at 140 V followed by transfer of protein to PVDF membrane (IPVH00010, Millipore) at 0.4 A. Blots were blocked by 3% BSA in TBST (25 m m Tris-HCl, pH 7.6, 150 m m NaCl, and 0.05% Tween 20) at room temperature for 20 min and then incubated with primary antibody at 4 °C overnight. .. After washing with TBST five times for 5 min each time, blots were incubated with HRP-conjugated secondary antibody for 1 h at room temperature followed by TBST washes again and developed using chemiluminescent substrate (E2400, Denville Scientific).
Western Blot:Article Title: O -Linked N -Acetylglucosamine ( O -GlcNAc) Transferase and O -GlcNAcase Interact with Mi2β Protein at the A γ-Globin Promoter
Article Snippet: .. Immunoblotting All electrophoresis was performed with 4–15% gradient Criterion TGX precast gels (567-1084, Bio-Rad) at 140 V followed by transfer of protein to PVDF membrane (IPVH00010, Millipore) at 0.4 A. Blots were blocked by 3% BSA in TBST (25 m m Tris-HCl, pH 7.6, 150 m m NaCl, and 0.05% Tween 20) at room temperature for 20 min and then incubated with primary antibody at 4 °C overnight. .. After washing with TBST five times for 5 min each time, blots were incubated with HRP-conjugated secondary antibody for 1 h at room temperature followed by TBST washes again and developed using chemiluminescent substrate (E2400, Denville Scientific).
Article Title: O-Linked N-Acetylglucosamine (O-GlcNAc) Transferase and O-GlcNAcase Interact with Mi2β Protein at the Aγ-Globin Promoter
Article Snippet: .. Immunoblotting: All electrophoresis was performed with 4-15% gradient Criterion TGX Precast Gels (567-1084, Bio-Rad) at 140 volts, followed by transferring protein to PVDF membrane (IPVH00010, Millipore) at 0.4 Amps. ..
Electrophoresis:Article Title: O -Linked N -Acetylglucosamine ( O -GlcNAc) Transferase and O -GlcNAcase Interact with Mi2β Protein at the A γ-Globin Promoter
Article Snippet: .. Immunoblotting All electrophoresis was performed with 4–15% gradient Criterion TGX precast gels (567-1084, Bio-Rad) at 140 V followed by transfer of protein to PVDF membrane (IPVH00010, Millipore) at 0.4 A. Blots were blocked by 3% BSA in TBST (25 m m Tris-HCl, pH 7.6, 150 m m NaCl, and 0.05% Tween 20) at room temperature for 20 min and then incubated with primary antibody at 4 °C overnight. .. After washing with TBST five times for 5 min each time, blots were incubated with HRP-conjugated secondary antibody for 1 h at room temperature followed by TBST washes again and developed using chemiluminescent substrate (E2400, Denville Scientific).
Article Title: O-Linked N-Acetylglucosamine (O-GlcNAc) Transferase and O-GlcNAcase Interact with Mi2β Protein at the Aγ-Globin Promoter
Article Snippet: .. Immunoblotting: All electrophoresis was performed with 4-15% gradient Criterion TGX Precast Gels (567-1084, Bio-Rad) at 140 volts, followed by transferring protein to PVDF membrane (IPVH00010, Millipore) at 0.4 Amps. ..
Article Title: O -Linked N -Acetylglucosamine ( O -GlcNAc) Transferase and O -GlcNAcase Interact with Mi2β Protein at the A γ-Globin Promoter
Article Snippet: .. All electrophoresis was performed with 4–15% gradient Criterion TGX precast gels (567-1084, Bio-Rad) at 140 V followed by transfer of protein to PVDF membrane (IPVH00010, Millipore) at 0.4 A. Blots were blocked by 3% BSA in TBST (25 m m Tris-HCl, pH 7.6, 150 m m NaCl, and 0.05% Tween 20) at room temperature for 20 min and then incubated with primary antibody at 4 °C overnight. .. After washing with TBST five times for 5 min each time, blots were incubated with HRP-conjugated secondary antibody for 1 h at room temperature followed by TBST washes again and developed using chemiluminescent substrate (E2400, Denville Scientific).
Transferring:Article Title: O-Linked N-Acetylglucosamine (O-GlcNAc) Transferase and O-GlcNAcase Interact with Mi2β Protein at the Aγ-Globin Promoter
Article Snippet: .. Immunoblotting: All electrophoresis was performed with 4-15% gradient Criterion TGX Precast Gels (567-1084, Bio-Rad) at 140 volts, followed by transferring protein to PVDF membrane (IPVH00010, Millipore) at 0.4 Amps. ..
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